micom.media =========== .. py:module:: micom.media .. autoapi-nested-parse:: Manages functions for growth media analysis and manipulation. Functions --------- .. autoapisummary:: micom.media.add_linear_obj micom.media.add_mip_obj micom.media.safe_weight micom.media.weight micom.media.minimal_medium micom.media.complete_medium Module Contents --------------- .. py:function:: add_linear_obj(community, exchanges, weights) Add a linear version of a minimal medium to the community. Changes the optimization objective to finding the growth medium requiring the smallest total import flux:: minimize sum w_i|r_i| for r_i in import_reactions :param community: The community to modify. :type community: micom.Community :param exchanges: The reactions to constrain. :type exchanges: list of cobra.Reaction :param weights: Maps each exchange reaction to a weight that is used in the minimization. :type weights: dict .. py:function:: add_mip_obj(community, exchanges) Add a mixed-integer version of a minimal medium to the community. Changes the optimization objective to finding the medium with the least components:: minimize size(R) where R part of import_reactions :param community: The community to modify. :type community: micom.Community :param exchanges: The reactions to constrain. :type exchanges: list of cobra.Reaction .. py:function:: safe_weight(met) Get the weight of a molecule. .. py:function:: weight(exchanges, what) Obtain elemental weights for metabolites. .. py:function:: minimal_medium(community, community_growth, min_growth=0.0, exports=False, exchanges=None, minimize_components=False, open_exchanges=False, solution=False, weights=None, atol=None, rtol=None) Find the minimal growth medium for the community. Finds the minimal growth medium for the community which allows for community as well as individual growth. Here, a minimal medium can either be the medium requiring the smallest total import flux or the medium requiring the least components (ergo ingredients). :param community: The community to modify. :type community: micom.Community :param community_growth: The minimum community-wide growth rate. :type community_growth: positive float :param min_growth: The minimum growth rate for each individual in the community. Either a single value applied to all individuals or one value for each. :type min_growth: positive float or array-like object. :param exports: Whether to include export fluxes in the returned medium. Defaults to False which will only return import fluxes. :type exports: boolean :param exchanges: The list of exchange reactions that are penalized. :type exchanges: list of cobra.Reactions :param minimize_components: Whether to minimize the number of components instead of the total import flux. Might be more intuitive if set to True but may also be slow to calculate for large communities. :type minimize_components: boolean :param open_exchanges: Whether to ignore currently set bounds and make all exchange reactions in the model possible. If set to a number all exchange reactions will be opened with (-number, number) as bounds. :type open_exchanges: boolean or number :param solution: Whether to also return the entire solution and all fluxes for the minimal medium. :type solution: boolean :param weights: Will scale the fluxes by a weight factor. Can either be "mass" which will scale by molecular mass, a single element which will scale by the elemental content (for instance "C" to scale by carbon content). If None every metabolite will receive the same weight. Will be ignored if `minimize_components` is True. :type weights: str :param atol: Absolute tolerance for the growth rates. If None will use the solver tolerance. :type atol: float :param rtol: Relative tolerqance for the growth rates. If None will use the solver tolerance. :type rtol: float :returns: A series {rid: flux} giving the import flux for each required import reaction. If `solution` is True retuns a dictionary {"medium": pandas.Series, "solution": micom.CommunitySolution}. If the medium optimization failed (usually because the specified growth rates are not reachable) it will return None. :rtype: pandas.Series, dict or None .. py:function:: complete_medium(model, medium, growth=0.1, min_growth=0.001, max_import=1, minimize_components=False, weights=None, strict=[]) Fill in missing components in a growth medium. Finds the minimal number of additions to make a model form biomass. In order to avoid bias all added reactions will have a maximum import rate of `max_import`. .. note:: This function fixes the growth medium for a single cobra Model. We also provide a function `complete_db_medium` in `micom.workflows` that fixes a growth medium for an entire model database. :param model: The model to use. :type model: cobra.Model :param medium: A growth medium. Must contain positive floats as elements and exchange reaction ids as index. Note that reactions not present in the model will be removed from the growth medium. :type medium: pandas.Series :param growth: The minimum overall growth rate that has to be achieved. For single COBRA model this is just the biomass flux and for community models this is the community biomass flux. :type growth: positive float :param min_growth: The minimum growth rate for each individual in the community. Either a single value applied to all individuals or one value for each. Only used if model is a `micom.Community` model. :type min_growth: positive float or array-like object. :param minimize_components: Whether to minimize the number of components instead of the total import flux. Might be more intuitive if set to True but may also be slow to calculate for large communities. :type minimize_components: boolean :param max_import: The import rate applied for the added exchanges. :type max_import: positive float :param weights: Will scale the fluxes by a weight factor. Can either be "mass" which will scale by molecular mass, a single element which will scale by the elemental content (for instance "C" to scale by carbon content). If None every metabolite will receive the same weight. Will be ignored if `minimize_components` is True. :type weights: str :param strict: strict : list Whether to match the imports in the predefined medium exactly. For reactions IDs listed here will not allow additional import of the components in the provided medium. For example, if your input medium has a flux of 10 mmol/(gDW*h) defined and the requested growth rate can only be fulfilled by ramping this up that would be allowed in non-strict mode but forbidden in strict mode. To match all medium components to strict mode use `strict=medium.index`. :type strict: list :returns: A series {rid: flux} giving the import flux for each required import reaction. This will include the initial `medium` as passed to the function as well as a minimal set of additional changes such that the model produces biomass with a rate >= `min_growth`. :rtype: pandas.Series or dict